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-   -   There are a lot of smart folks that frequent this venue, maybe one of you will know (http://forums.pelicanparts.com/off-topic-discussions/1099367-there-lot-smart-folks-frequent-venue-maybe-one-you-will-know.html)

Tobra 08-05-2021 08:13 PM

There are a lot of smart folks that frequent this venue, maybe one of you will know
 
What is in a PCR test swab that can't tolerate heat?

Ayles 08-05-2021 08:45 PM

https://www.houstonchronicle.com/techburger/amp/How-to-tell-a-Russian-troll-from-a-regular-person-13205901.php

black73 08-06-2021 02:26 AM

Coronavirus.

hbueno 08-06-2021 03:21 AM

Magnetic particles.

porsche4life 08-06-2021 07:37 AM

Are you ****ing serious? You as a medical professional don’t understand why some types of tests or samples need to be refrigerated?

nota 08-06-2021 08:40 AM

Because temperature control can be a notable factor in point-of-care assays, we next analyzed the effect of lysis temperature on CRISPR-FDS signal. No signal was detected when lysis reactions were performed at room temperature (~22°C), similar CRISPR-FDS signal was detected at 37° and 65°C, the manufacturer-recommended lysis temperature, with the most signal detected at 95°C (Fig. 1C). We next examined the effect of lysis time on CRISPR-FDS signal when using a 1:1 saliva-to-buffer ratio at 65°C and found that signal increased with incubation time out to the final 15-min time point (Fig. 1D). Given that there was no difference between 65° and 37°C lysis, we elected to use 37°C for the lysis temperature, with the assumption that this minimum temperature would be the simplest to achieve, maintain, and measure in most point-of-care settings, although temperature deviations above this minimum would not negatively influence the lysis reaction. We also chose to use a 5-min lysis time to minimize overall assay performance time.

FROM

https://advances.sciencemag.org/content/7/2/eabe3703

fintstone 08-06-2021 10:17 AM

Nothing.

masraum 08-06-2021 10:22 AM

Quote:

Originally Posted by porsche4life (Post 11415079)
Are you ****ing serious? You as a medical professional don’t understand why some types of tests or samples need to be refrigerated?

My guess is he's looking for something more specific than "some tests need it" and he's not a geneticist.

fintstone 08-06-2021 10:51 AM

Don't samples require refrig...but not the swab?

Ayles 08-06-2021 11:10 AM

^ Also not a geneticist

Sooner or later 08-06-2021 11:12 AM

Once the sample is taken it has temperature requirements. Doesn't seem to have any temperature requirement for the swab itself.

https://www.cdc.gov/coronavirus/2019-ncov/lab/guidelines-clinical-specimens.html

fintstone 08-06-2021 11:15 AM

Quote:

Originally Posted by Ayles (Post 11415383)
^ Also not a geneticist

^Also not a genius

Yep...only Geneticists store or use PCR swabs...LOL. It is usually the lowest level technician on staff.

Do you need an automotive engineer to add oil to your car?

Tobra 08-07-2021 11:35 AM

What do you want to be if you grow up Ayles?

Quote:

Originally Posted by porsche4life (Post 11415079)
Are you ****ing serious? You as a medical professional don’t understand why some types of tests or samples need to be refrigerated?

Generally this is an issue after the culture is done, not before. Did you even read the first post in this thread at all?

Next time you are looking at something sterile, inspect the packaging and it will have an EO on it to indicate ethylene oxide was used. Pretty much everything is heat sterilized if it can be, for a number of reasons, primarily expense.
Quote:

Originally Posted by Sooner or later (Post 11415387)
Once the sample is taken it has temperature requirements. Doesn't seem to have any temperature requirement for the swab itself.

https://www.cdc.gov/coronavirus/2019-ncov/lab/guidelines-clinical-specimens.html

Exactly

island911 08-07-2021 11:44 AM

Quote:

Originally Posted by Tobra (Post 11416433)
What do you want to be if you grow up Ayles?
..

c'mon Tobra, be realistic. Clearly he doesn't want to grow up.

island911 08-07-2021 11:48 AM

Quote:

Originally Posted by Tobra (Post 11414673)
What is in a PCR test swab that can't tolerate heat?

Quote:

Originally Posted by black73 (Post 11414783)
Coronavirus.

Wow. I did not know that.

Hey, if the Coronavirus cannot tolerate heat, how does it survive at 98.6F ?

Or does body heat kill it instantly?


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