There are a lot of smart folks that frequent this venue, maybe one of you will know

[Tobra]

What is in a PCR test swab that can't tolerate heat?

[Ayles]

https://www.houstonchronicle.com/techburger/amp/How-to-tell-a-Russian-troll-from-a-regular-person-13205901.php

[black73]

Coronavirus.

[hbueno]

Magnetic particles.

[porsche4life]

Are you ****ing serious? You as a medical professional don’t understand why some types of tests or samples need to be refrigerated?

[nota]

Because temperature control can be a notable factor in point-of-care assays, we next analyzed the effect of lysis temperature on CRISPR-FDS signal. No signal was detected when lysis reactions were performed at room temperature (~22°C), similar CRISPR-FDS signal was detected at 37° and 65°C, the manufacturer-recommended lysis temperature, with the most signal detected at 95°C (Fig. 1C). We next examined the effect of lysis time on CRISPR-FDS signal when using a 1:1 saliva-to-buffer ratio at 65°C and found that signal increased with incubation time out to the final 15-min time point (Fig. 1D). Given that there was no difference between 65° and 37°C lysis, we elected to use 37°C for the lysis temperature, with the assumption that this minimum temperature would be the simplest to achieve, maintain, and measure in most point-of-care settings, although temperature deviations above this minimum would not negatively influence the lysis reaction. We also chose to use a 5-min lysis time to minimize overall assay performance time.

FROM

https://advances.sciencemag.org/content/7/2/eabe3703

[fintstone]

Nothing.

[masraum]

Originally Posted by porsche4life

Are you ****ing serious? You as a medical professional don’t understand why some types of tests or samples need to be refrigerated?

My guess is he's looking for something more specific than "some tests need it" and he's not a geneticist.

[fintstone]

Don't samples require refrig...but not the swab?

[Ayles]

^ Also not a geneticist

[Sooner or later]

Once the sample is taken it has temperature requirements. Doesn't seem to have any temperature requirement for the swab itself.

https://www.cdc.gov/coronavirus/2019-ncov/lab/guidelines-clinical-specimens.html

[fintstone]

Originally Posted by Ayles

^ Also not a geneticist

^Also not a genius

Yep...only Geneticists store or use PCR swabs...LOL. It is usually the lowest level technician on staff.

Do you need an automotive engineer to add oil to your car?

[Tobra]

What do you want to be if you grow up Ayles?

Originally Posted by porsche4life

Are you ****ing serious? You as a medical professional don’t understand why some types of tests or samples need to be refrigerated?

Generally this is an issue after the culture is done, not before. Did you even read the first post in this thread at all?

Next time you are looking at something sterile, inspect the packaging and it will have an EO on it to indicate ethylene oxide was used. Pretty much everything is heat sterilized if it can be, for a number of reasons, primarily expense.

Originally Posted by Sooner or later

Once the sample is taken it has temperature requirements. Doesn't seem to have any temperature requirement for the swab itself.

https://www.cdc.gov/coronavirus/2019-ncov/lab/guidelines-clinical-specimens.html

Exactly

[island911]

Originally Posted by Tobra

What do you want to be if you grow up Ayles?
..

c'mon Tobra, be realistic. Clearly he doesn't want to grow up.

[island911]

Originally Posted by Tobra

What is in a PCR test swab that can't tolerate heat?

Originally Posted by black73

Coronavirus.

Wow. I did not know that.

Hey, if the Coronavirus cannot tolerate heat, how does it survive at 98.6F ?

Or does body heat kill it instantly?